colorimetric kit Search Results


96
Dojindo Labs iron assay kit
Iron Assay Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology iron assay kit
Iron Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology atp assay kit
Atp Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology e bc k130 s
E Bc K130 S, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology cell total iron colorimetric assay kit
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Elabscience Biotechnology antioxidant capacity t aoc colorimetric measurement kit
Antioxidant Capacity T Aoc Colorimetric Measurement Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology bca protein assay kit
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Elabscience Biotechnology malondialdehyde mda
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Elabscience Biotechnology ferrous iron colorimetric assay kit
Ferrous Iron Colorimetric Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology lactic acid
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Elabscience Biotechnology nad nadh colorimetric assay kit wst 8
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Elabscience Biotechnology nadp nadph assay kit
LINC01532 is identified as a potential <t>NADPH-metabolism-related</t> lncRNA in HCC drug resistance. ( A ) The expression of G6PD, ME1, ME2 and MTHFD2 was analyzed in HCC tissues and adjacent non-cancerous tissues using TCGA dataset. ( B ) Venn diagram was employed to analyze the overlap lncRNAs among G6PD-related lncRNAs, prognosis related lncRNAs and differentially expressed lncRNAs. ( C ) The expression of the eight lncRNAs was evaluated by RT-qPCR in HCC tissues and the adjacent non-tumoral tissues from our hospital. ( D ) HuH-7 cells were transfected with indicated siRNAs and cellular NADPH level was determined. ( E ) The expression of eight lncRNAs was evaluated by RT-qPCR in lenvatinib-resistant HuH-7 cells. ( F ) The LINC01532 expression was analyzed in HCC tissues and adjacent non-tumoral tissues. ( G ) Overall survival (OS) and disease-free survival (DFS) analyses based on LINC01532 levels were analyzed using data from TCGA-LIHC cohort. ( H ) OS and DFS analyses based on LINC01532 level were analyzed using data from our cohort. ( I ) RT-qPCR was performed to detect LINC01532 expression in the cytoplasmic and nuclear fractions. ( J ) The subcellular localization of LINC01532 was determined by Fluorescence in situ hybridization (FISH). Scale bar, 10 μm. ( K ) LINC01532 coding capability was performed by CPAT ( http://lilab.research.bcm.edu ).
Nadp Nadph Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


LINC01532 is identified as a potential NADPH-metabolism-related lncRNA in HCC drug resistance. ( A ) The expression of G6PD, ME1, ME2 and MTHFD2 was analyzed in HCC tissues and adjacent non-cancerous tissues using TCGA dataset. ( B ) Venn diagram was employed to analyze the overlap lncRNAs among G6PD-related lncRNAs, prognosis related lncRNAs and differentially expressed lncRNAs. ( C ) The expression of the eight lncRNAs was evaluated by RT-qPCR in HCC tissues and the adjacent non-tumoral tissues from our hospital. ( D ) HuH-7 cells were transfected with indicated siRNAs and cellular NADPH level was determined. ( E ) The expression of eight lncRNAs was evaluated by RT-qPCR in lenvatinib-resistant HuH-7 cells. ( F ) The LINC01532 expression was analyzed in HCC tissues and adjacent non-tumoral tissues. ( G ) Overall survival (OS) and disease-free survival (DFS) analyses based on LINC01532 levels were analyzed using data from TCGA-LIHC cohort. ( H ) OS and DFS analyses based on LINC01532 level were analyzed using data from our cohort. ( I ) RT-qPCR was performed to detect LINC01532 expression in the cytoplasmic and nuclear fractions. ( J ) The subcellular localization of LINC01532 was determined by Fluorescence in situ hybridization (FISH). Scale bar, 10 μm. ( K ) LINC01532 coding capability was performed by CPAT ( http://lilab.research.bcm.edu ).

Journal: Journal of Advanced Research

Article Title: Long non-coding RNA LINC01532 sustains redox homeostasis and accelerates lenvatinib resistance in hepatocellular carcinoma

doi: 10.1016/j.jare.2025.02.035

Figure Lengend Snippet: LINC01532 is identified as a potential NADPH-metabolism-related lncRNA in HCC drug resistance. ( A ) The expression of G6PD, ME1, ME2 and MTHFD2 was analyzed in HCC tissues and adjacent non-cancerous tissues using TCGA dataset. ( B ) Venn diagram was employed to analyze the overlap lncRNAs among G6PD-related lncRNAs, prognosis related lncRNAs and differentially expressed lncRNAs. ( C ) The expression of the eight lncRNAs was evaluated by RT-qPCR in HCC tissues and the adjacent non-tumoral tissues from our hospital. ( D ) HuH-7 cells were transfected with indicated siRNAs and cellular NADPH level was determined. ( E ) The expression of eight lncRNAs was evaluated by RT-qPCR in lenvatinib-resistant HuH-7 cells. ( F ) The LINC01532 expression was analyzed in HCC tissues and adjacent non-tumoral tissues. ( G ) Overall survival (OS) and disease-free survival (DFS) analyses based on LINC01532 levels were analyzed using data from TCGA-LIHC cohort. ( H ) OS and DFS analyses based on LINC01532 level were analyzed using data from our cohort. ( I ) RT-qPCR was performed to detect LINC01532 expression in the cytoplasmic and nuclear fractions. ( J ) The subcellular localization of LINC01532 was determined by Fluorescence in situ hybridization (FISH). Scale bar, 10 μm. ( K ) LINC01532 coding capability was performed by CPAT ( http://lilab.research.bcm.edu ).

Article Snippet: NADPH, G6PD, GSH and MDA levels were evaluated using NADP + /NADPH Assay Kit (Beyotime, China), G6PDH Activity Assay Kit (Beyotime, China), Reduced Glutathione Content Assay Kit (Solarbio, China) and Malondialdehyde (MDA) Colorimetric Assay Kit (Elabscience, China), respectively, based on the manufacturer's protocol.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Fluorescence, In Situ Hybridization

LINC01532 elevates PPP flux. ( A ) Cellular NADPH level was determined in HuH-7 and MHCC97-H stably overexpressing LINC01532. ( B ) Cellular G6PD activity was evaluated in the indicated cells. ( C ) Cellular NADPH level was detected in the indicated cells. ( D ) Cellular G6PD activity was determined in the indicated cells. ( E ) Cellular GSH level was assessed in HuH-7 and MHCC97-H stably overexpressing LINC01532. ( F ) Cellular GSH level was analyzed in the indicated cells. ( G ) Oil red O staining assay (left) was performed in the indicated cells. The numbers of lipid droplets around cells were summarized (n = 50, right). ( H ) Cellular NADPH level was determined in the indicated cells treated with RRX-001. ( I ) The indicated cells were treated with RRX-001 and cellular GSH level was determined. ( J ) Cellular NADPH level was determined in the cells transfected as indicated. ( K ) Oil red O staining (left) and statistical analysis (right) were performed in HuH-7 cells as indicated.

Journal: Journal of Advanced Research

Article Title: Long non-coding RNA LINC01532 sustains redox homeostasis and accelerates lenvatinib resistance in hepatocellular carcinoma

doi: 10.1016/j.jare.2025.02.035

Figure Lengend Snippet: LINC01532 elevates PPP flux. ( A ) Cellular NADPH level was determined in HuH-7 and MHCC97-H stably overexpressing LINC01532. ( B ) Cellular G6PD activity was evaluated in the indicated cells. ( C ) Cellular NADPH level was detected in the indicated cells. ( D ) Cellular G6PD activity was determined in the indicated cells. ( E ) Cellular GSH level was assessed in HuH-7 and MHCC97-H stably overexpressing LINC01532. ( F ) Cellular GSH level was analyzed in the indicated cells. ( G ) Oil red O staining assay (left) was performed in the indicated cells. The numbers of lipid droplets around cells were summarized (n = 50, right). ( H ) Cellular NADPH level was determined in the indicated cells treated with RRX-001. ( I ) The indicated cells were treated with RRX-001 and cellular GSH level was determined. ( J ) Cellular NADPH level was determined in the cells transfected as indicated. ( K ) Oil red O staining (left) and statistical analysis (right) were performed in HuH-7 cells as indicated.

Article Snippet: NADPH, G6PD, GSH and MDA levels were evaluated using NADP + /NADPH Assay Kit (Beyotime, China), G6PDH Activity Assay Kit (Beyotime, China), Reduced Glutathione Content Assay Kit (Solarbio, China) and Malondialdehyde (MDA) Colorimetric Assay Kit (Elabscience, China), respectively, based on the manufacturer's protocol.

Techniques: Stable Transfection, Activity Assay, Staining, Transfection